EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Visualization: Immuno-Fixation

Mai 30, 2018

This Immuno-Fixation is followed by the ammoniacal silver staining. Immuno-fixation and silver staining simply done inside the gel. The silver staining starts from step 5, no TCA-fixing and washing necessary.
This method combines the sensitivity of the silver-staining with the specificity of the antibody-reaction.

Advantage: Only IgG will be stained, band-sharpness and sensitivity as good as with the general silver-staining.
Disadvantage: One overnight step (washing out of the anti-human IgG serum)

Gelreh

The Immunoreaction is done in a special saoking pool in 30 ml (100 ul Antibody), 2 hours slightly rocked

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pfeil45
Stainshk

Overnight: Washing out exceed amount of the antibody

autostainklein

Silver staining can be automated with GE's Autostainer

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pH611kurz
Immunfix Silver Stain

Gel: IEFGel pH 6-11
Visualization: Immuno-Fixation followed by silver staining
(Courtesy of G.Cox, Charlton Memorial Hospital, Swansea)