EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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IEF-Standards

Dilutions* of the standards are given when as a visualization process Coomassie-staining is chosen. For silver-staining dilute further 1:25.

Product

Dilution*
(marker + buffer)

Prestained ?

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Order Number

GE 3-10 pH

200 ul per bottle

---

1 ug in 25 ul

17-0471-01

Biorad 4.65 - 9.6

10 ul + 240 ul

YES

1 ug in 25 ul

161-0310

Native Protein Mix
(see below)

150 ul + 150 ul

Myoglobin,
Cytochrom C

~ 1.3 ug in 25 ul

- self mix -

Mai 30, 2018

Native Protein-Mix:  each 1 mg / 10 ml. Can be used in both directions, anodal and cathodal. Should look like GE’s IEF Mix 3-10.
Amyloglucosidase - pI 3.5, Glucose oxidase - pI 4.2, Trypsin inhibitor - pI 4.5, β-Lactoglobulin - pI 5.15 / 5.3
Carboanhydrase  - pI 6.0, Myoglobin horse - pI 6.9 / 7.35, Lentil lectin - pI 7.75 / 8.0 / 8.3, Ribonuclease A - pI 9.45
Cytochrome C - pI 10.65

IEFGel 3-10, Coomassie-Violet stained
Standard: GE 3-10

Amersham3-10Klein

IEFGel 3-10, during run
BioRad 4.6-9.6

BioRadMarkerIEFKlein