EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Direct Dye Staining

Mai 30, 2018

The target molecules were stained directly by reacting them with a dye-molecule.
Very easy procedure, quantification is possible; not the most sensitive method!

[Prot-Dye]
[DNA-Dye]

Coomassie R & G, Coomassie Violet

Stains All

Molecule

Dye

Sensitivity*

Info

proteins

Coomassie R 350 Sigma B4921-20TAB*
Coomassie-Violet 17 Sigma #210579-50g

10-100 ng

...silver-staining can follow!

DNA

“Stains All” Sigma # E9379-1G

10 ng

...silver-staining can follow!

Staining
Pfeil

Hot staining procedure

* per band, * 0,4 g dye per tablet

MultiWash-Destainer

...destaining procedure for Coomassie

Pictures from the book “Electrophoresis in Practise” by Dr. R. Westermeier


Stains all” is the most simple DNA staining procedure and quantifiable!