When the first dimension is a IEF (isoelectric focusing) and the second dimension is a native electrophoresis (anodal or cathodal - instead of the SDS-dimension of a 2D), this combination is called “Double Native Electrophoresis”.
First dimension is an IEF
Second dimension is an anodal native
Mai 30, 2018
The second dimension does not display the molweights of the proteins, but 2 proteins with the same isoelectric point and different secondary or tertiary structure will be separated.