EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Double Native Electrophoresis

When the first dimension is a IEF (isoelectric focusing) and the second dimension is a native electrophoresis (anodal or cathodal - instead of the SDS-dimension of a 2D), this combination is called “Double Native Electrophoresis”.

First dimension is an IEF

double native

Second dimension is an anodal native

Mai 30, 2018

The second dimension does not display the molweights of the proteins, but 2 proteins with the same isoelectric point and different secondary or tertiary structure will be separated.

double nativ - molweights