EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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DNA-Electrophoresis Trouble Shooting

Mai 30, 2018

Gel is leaving its support film:
Do not use staining recipes with alcohol concentrations higher than 40%.
The gel will shrink or swell to much so the forces between the film and the gel get to high!

Troubles-MeOH

Not many things can be done wrong in DNA-fragment electrophoresis!
Most frequently done mistakes:

1.   Wrong concentrated samples
2.    Samples are cut off on the anodal end of the gel
2.   Wrong resolution

[Concentration]
[Cut-Off]
[Resolution]