EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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DNA-Standards

This is a list of different DNA-standards

Mai 30, 2018

Product

Dilution Silver native
(marker + buffer***)

Load****
(per band)

Dilution Silver denat
(marker + 50% Formamide**)

Load****
(per band)

Dilution Fluorescence
(native)

Load****
(per band)

Dilution Direct Stain
(marker + buffer***)

Load****
(per band)

Dilution Direct Stain denat
(marker + 50% Formamide**)

Load****
(per band)

Order #

Promega 100 bp

 30 ul + 720 ul  

2.5 ng in 6 ul

 10 ul + 140 ul

5 ng in 6 ul

not good!

 

10 + 10

60 ng in 6 ul

 

 

G210A

Invitrogen 50 bp

10 ul + 990 ul

3 ng in 6 ul

10 ul + 300 ul

10 ng in 6 ul

10 + 190

15 ng in 6 ul

10 + 90

35 ng in 6 ul

10 + 40

75 ng in 6 ul

10416-014

Cambrex 20 bp

40 ul + 500 ul

4 ng in 6 ul

not good!

 

not good!

 

10 + 15

40 ng in 6 ul

 

 

50320

Invitrogen 10 bp

10 ul + 700 ul

--

 10 ul + 200 ul

--

not good!

 

10 + 40

--

10 + 20

--

10821-015

Amersham 100 bp

 10 ul + 1290 ul

 

not good!

 

10 + 240

 

not tested

 

 

 

27-407-01

Amersham 1kb

10 ul + 990 ul

 

not good!

 

10 + 190

 

not tested

 

 

 

27-404-01

Promega Hinf 1

 10 ul + 1840 ul

 

10 ul + 740 ul

 

not good!

 

not tested

 

 

 

G1751

Roth Quant 200

10 ul + 590 ul

10 ng in 6 ul

not yet tested!

 

10 + 120

 

10 ul + 90 ul

60 ng in 6 ul

20 ul + 80 ul

120 ng in 6 ul

QS200

****Load: According to the manufactor's figures in the manuals.
***
Native:
The dilution buffer should be the gel-buffer + 0.005% Xylene Cyanol.
**Denaturing: The dilution solution should be 50% Formamide + 0.005% Xylene Cyanol.
*10% (v/v) Dye: The fluorescence dye-solution should be diluted with DMSO.
Results: The markers should be visible easily in the above given dilution. Please see the native separation and the denaturing result below.

Silver staining: Gel 15%T run native
with a special buffer pH 7.4. About 3 ng band

benzgood

Silver staining: Gel 15%T run denaturing*
with a special buffer pH 8.3, containing 6 M Urea.
About 3 ng per band

Etcrot

Fluorescence: Gel 10%T, run native
with a special buffer pH 8.3, staining with GelStar. About 50 ng per band

Direct DNA stain: Gel 10%T, run native with a special buffer pH 7.4,
staing with Stains All. Concentrated lanes hold about 30 ng per band

Direct DNA-Stain: Gel 15%T, run semidisc denatured
with a special buffer pH 8.3, staining with Stains All. About 60 ng per band.