EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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DNA-Silver Staining Trouble Shooting

Mai 30, 2018

Due to their phosphate-groups the DNA-fragments are heavily negative charged molecules.
Because of that the silver-staining protocols are normally short and the perfomancesare without any problems.
But one mistake should not be done:
The first step, the fixing procedure, should not be to weak and/or to short:
The smaller DNA-fragments will then be not immobilized enough and they will be not in the gel anymore when the development step is performed. See the 2 pictures below.
Additional advantage is that recipes with this fixing step will stain all gel-types also these including 7 M Urea!

Fixing with HAc/EtOH, 5 min

stain1

Fixing with Benzenesulfonic Acid, 20 min

stain3