EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Anodal Cut-Off

Mai 30, 2018

Most electrophoretic buffer-systems are discontinuous buffer-systems. This sharpens the bands. But as a side-effect some of the smaller fragments (15%-gel: <30 bp) are taken away with the discontinuous front.
The semidisc method softens this effect: The bands are a little bit more unsharp, but the anodal cut-off is avoided. Take 10% of the electrode buffer to the gel-buffer

Discontinuous run: The 3 samples are cut off at the anodal end of the 15% gel

DnaTrouble#4
pfeil9002

Semidisc run: The 3 samples are within the gel

DnaTrouble#5