EDC Impressum & DSE

Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Curved bands

Mai 30, 2018

Symptom: Bands occur curved within their lanes.
Cause: When samples hold too much salt and/or buffer-ions they change the gel's conductivity in that lane. The isoelectric lines together with the proteins will be no more parallel.
1.   Try another sample application points.
2.   Desalt sample using small columns like NAP-5 (GE 17-0853-01)

No curved bands at neutral sample application point

Curved bands at acidic sample
application point