EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Curved bands

Mai 30, 2018

Symptom: Bands occur curved within their lanes.
Cause: When samples hold too much salt and/or buffer-ions they change the gel's conductivity in that lane. The isoelectric lines together with the proteins will be no more parallel.
Remedy:
1.   Try another sample application points.
2.   Desalt sample using small columns like NAP-5 (GE 17-0853-01)

No curved bands at neutral sample application point

Curved bands at acidic sample
application point