EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Coomassie Staining Troubles of SDS-Gels and Native Gels

Mai 30, 2018

Peptides
Cause:
Peptides are not easy to stain: Normally they leave the gel during visualisation if there is any solvent like 20-30% ethanol or methanol.
Remedy: The “Semi-Colloidal Coomassie” and the “Hot Coomassie” is staining and fixing at the same time and has no organic solvents.

GE Peptide-marker and bacterium-lysate on a 1-dimensionalo SDS-gel 15%
K.Buettner, Greifswald

PeptidemikrogrammKlein