EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Coomassie Staining Troubles of 2D gels

Mai 30, 2018

The problem of staining second dimensions of a 2D electrophoresis:
To achieve better focusing, Pharmalytes or Biolytes are mixed to the IPG-strip’s rehydration solution.
Together with the SDS-molecule nearly unsoluble complexes appear mainly in the basic region. See the picture below and the trouble shooting page: 1D-Ampholyte
Please see the special notes on the suggested staining methods below

pharmalyte_sds_klein

“Blue Curtain” in the basic region

[1D-no Pharmalytes]
[1D-plus Pharmalytes]