Concentration ranges in DNA-electrophoresis are depending on the visualization procedure, but there is also a upper limit (when the DNA-molecules determine the local pH-value, not the buffering molecules)
Range (per band)
10 ng - 500 ng
500 pg - 250 ng
100 pg - 250 ng
After silver-stain: Markers are at 10 ng per band The 3 samples are over 3 ug!
After silver-stain: The 3 samples are diluted: 1:10 - 1:30 - 1:50 - 1:100 Silver-stain is not neaded!