EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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DNA-Concentration

Mai 30, 2018

Concentration ranges in DNA-electrophoresis are depending on the visualization procedure, but there is also a upper limit (when the DNA-molecules determine the local pH-value, not the buffering molecules)

Visualization

Range (per band)

Upper Limit

~3 ug

Direct Stain

10 ng - 500 ng

Fluorescence

500 pg - 250 ng

Silver Staining

100 pg - 250 ng

DnaTrouble#102
pfeil90

After silver-stain:
Markers are at 10 ng per band
The 3 samples are over 3 ug!

DnaTrouble#2

After silver-stain:
The 3 samples are diluted:
1:10 - 1:30 - 1:50 - 1:100
Silver-stain is not neaded!

Pfeil180
DnaTrouble3#3

Direct staining: convenient method!