EDC Impressum & DSE

Electrophoresis
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Dr. Hanspeter Schickle
Wolfgang Gstrein

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Blue (Coomassie-staining) or black (silver-staining) curtain in the alkaline region

How to avoid the Pharmalytes-SDS complexes to be stained after the second dimension?
Pharmalytes are optimal as addition in the first dimension. Here two remedies.....

Mai 30, 2018

Remedy #1: Stay with the Pharmalytes and use the additional washing steps given in the different staining methods: Hot Coomassie, Semi Colloidal Coomassie

Direct Coomassie Staining without the additional washing steps
Pfeil

Pharmalytes in the first dimension - Hot Coomassie Staining
without the additional washing steps

Hot Coomassie + additional washing steps

Pharmalytes in the first dimension - Hot Coomassie Staining
 + the additional washing steps

Remedy #2: Use Servalytes / Sinulytes in a lower concentration in the first dimension:

Pharmalyte in the first dimension
Pfeil

Pharmalyte-SDS complexes in the second dimension (left side), silver-staining started with TCA (Trichloroacetic Acid)

Sinulyte in the first dimension

Servalyte / Sinulyte / SepaLyte will not form SDS complexes in the second dimension