EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Acidic Peptides

Acidic peptides could be easily separated in IEF, native and in SDS-electrophoresis.

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E.coli in native electrophoresis

NativAnodal4SKlein

Native electrophoresis displays better at the anodal end.
Migration speed is not relative to the mol-weight!

E.coli in SDS-electrophoresis

SDSEcoliKlein

Peptides not only at the anodal end!

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Glyco-peptides (milk) in anodal native electrophoresis

webAnodisch7.4Glyco15prozKlein

Gel 15%T, native buffer pH 7.4.
Ingolf Krause (Weihenstephan, Germany)

Mai 30, 2018


Two dimensional electrophoresis beside the classical 2D:  double native 2D
First dimension = IPG-IEF pH 4-7, 11 cm
Second dimension = anodal native electrophoresis native buffer

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pH 4

-----IPG-strip 4-7-----

pH 7

DoubleNativeAnodal#4ASKlein

Double Native electrophoresis of 1 mg E.coli.
First dimension: IPG-strip pH 4-7, 11 cm
Second dimension: 12.5%R, native anodal buffer pH 7.4
Visualization: Hot Coomassie

migration speed is not relative to the molweight

DoubleNativeFalschfarben#2Klein