EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Too much Heat / Watt in the vertical second dimension

In the manuals are the running conditions given:

All 3 values have a limitation each: Volt - mA and Watt.
These values are written in the manuals!
Do not run the power supply only with one or two limitation values.
Don’t forget to input the Watt-limitations, normally all vertical second dimension gels run under Watt-limitations (Watt-controlled).

All upper buffer-tanks in all vertical systems are not cooled!

Mai 30, 2018

In the second phase the run continues running mA-constant.
But the electric resistance rises up further on due to the exchange of leading ions (in the gel) through following ions (from the buffer):

The power supply tries to compensate that by rising up the voltage. This increases of course the Watt (=mA x W x 1000) which is relative to the Joule’s heat producting in the gel.

This value have to be limited, normally at 15 Watt per vertical gel!

1 gel

set V

start V-value

set mA

set W

time

temp

phase 1

60

~30

10

0.5

1 h

25°

phase 2

600

~200

60

15

5 h 30 min

25°

The second dimension (vertical) is running too hot!

TSHeatWatt