EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Spots show backward striking

Mai 30, 2018

Uncomplete equilibration of the focused, native proteins.

Use special equilibration buffer coming with the buffer-kits.
Do not use “Thiourea”!
Use the step-times written in EDC’s manuals.

TSBackwardStreaking

Backward-striking

mit-bremsstreifen-klein
arrowgr

Thiourea in the equilibration buffers
(by C.Hentschker, Greifswald, Germany)

ohne-bremsstreifen-klein

No Thiourea in the equilibration buffers
(by C.Hentschker, Greifswald, Germany)