EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Hot Coomassie R-350 Stainings

Mai 30, 2018

No Pharmalytes in the first dimension:
For the hot staining processes a special stainless steel tray with grid is necessary

If Pharmalytes or Biolytes were rehydrated into the IPG-strip see the special note on the page describing this staining method trouble --> special page

Hot Coomassie Assembling

A steel tray with a grid

The staining procedures

[Hot-Coomassie] [Semi-Colloidal Coom]

Run horizontal on a 2DGel DryGel 12.5%, Hot Coomassie Staining

Half of a DryGel-2D with Hot Coomassie